Derivative Assay:Article Title: Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces
Article Snippet: Antibodies used in this study included the homemade affinity purified rabbit anti- T. gondii toxofilin [ ], affinity purified mouse anti- T. gondii RON4 antibodies [ ], mouse monoclonal anti -T. gondii P30 antibodies (Novocastra, Leica Biosystem, Nanterre, France), polyclonal rabbit anti-p34-Arc/ARPC2 (ref 07-227 batch 32474, Upstate, Millipore, Molsheim, France), mouse monoclonal anti-cortactin p80/p85 (clone 4 F11, ref 05-180, batch 28747, Upstate, Millipore, Molsheim, France), anti-non-muscle myosin II (ref M8064, Sigma-Aldrich, Lyon, France), mouse anti-Ty antibodies (ref 200-301-W45, Rockland Immunochemicals Inc., Limerick, PA, USA), anti-recombinant ROP2 serum (gift of J.F.
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35) (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis.
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35)23 (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Codependencies of mTORC1 signaling and endolysosomal actin structures
Article Snippet: 488 Goat Molecular Probes (A21206) 1/200 Anti-mouse Cy3 Donkey Jackson ImmunoResearch (715-165-151) 1/500 Anti-rabbit Cy3 Donkey Molecular Probes (A21206) 1/800 Anti-chicken Alexa Fluor 488 Donkey Molecular Probes (A11039) 1/300 Table S4.
Article Title: MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes
Article Snippet: The other antibodies used in this study were ARPC2/p34-Arc rabbit polyclonal (Millipore; 07–227, 1:1,000 dilution), ARPC5 mouse monoclonal (Synaptic Systems; 305011, clone 323H3, 1:1,000 dilution), Arp2 rabbit monoclonal (Abcam; ab129018, clone EPR7979, 1:1,000 dilution), ARPC5L rabbit monoclonal (Abcam; ab169763, clone EPR10274, 1:1,000 dilution), ARPC1A rabbit polyclonal (Sigma; HPA004334, 1:250 dilution), Arp3 mouse monoclonal (Sigma; A5979, clone FMS338, 1:1000 dilution), ARPC1B rabbit polyclonal (Bethyl Laboratories; A302-781, 1:1,000 dilution), ARPC3 mouse monoclonal (BD Transduction Laboratories; 612234, clone 26/p21-Arc, 1:1,000 dilution), ARPC4 rabbit polyclonal (Sigma; SAB1100901, 1:1,000 dilution), β-actin mouse monoclonal (Sigma; A5316, clone AC-74, 1:1,000 dilution), Vinculin mouse monoclonal (Sigma; V4505, 1:1000 dilution), coronin 1C rabbit polyclonal (Invitrogen; PA5-21775, 1:500 dilution), α-Actinin mouse monoclonal (Sigma; A5044, 1:1,000 dilution), GAPDH mouse monoclonal (Santa Cruz Biotechnology; sc-32233, 1:1,000 dilution), cortactin mouse monoclonal (Millipore; 05–180, clone 4F11, 1:1,000 dilution), MICAL1 rabbit polyclonal (Proteintech; 14818–1-AP, 1:1,000 dilution), GFP mouse monoclonal (custom made by Cancer Research UK; 1:1,000 dilution).
Article Title: Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes
Article Snippet: Antibody rabbit polyclonal IgG anti-NMIIA Biolegend P909801 Used at 1:1000 Antibody rabbit polyclonal IgG anti-NMIIB Cell Signaling P3404S Used at 1:200 Antibody rabbit polyclonal IgG anti-NMIIB Biolegend 909901 Used at 1:200 Antibody mouse monoclonal IgG anti-bCMII Iowa Hybridoma Bank A4.1025 Used at 1:2 Antibody rabbit polyclonal IgG anti-a-actinin-2 Sigma-Aldrich clone EA-53 Used at 1:200 Antibody rabbit polyclonal IgG anti-p34-Arc/ArpC2 (Arp2/3 complex) Millipore Sigma 07–227 Used at 1:100 Antibody rabbit polyclonal IgG anti-DAAM1 Bethyl Laboratories HPA026605 Used at 1:100 Antibody rabbit polyclonal IgG anti-DIAPH1 Sigma-Aldrich A300-078A Used at 1:100 Antibody mouse monoclonal IgGanti-cardiac troponin T Santa Cruz Biotechnology CT3, sc-20025 Used at 1:50 Antibody goat anti mouse IgG Alexa Fluor 568 ThermoFisher Scientific A-11004 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 568 ThermoFisher Scientific A-11011 Used at 1:100 Antibody goat anti mouse IgG Alexa Fluor 488 ThermoFisher Scientific AA28175 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 647 ThermoFisher Scientific A-21244 Used at 1:100 Antibody goat anti mouse IgM Alexa Fluor 488 ThermoFisher Scientific A-21042 Used at 1:100 Antibody mouse monoclonal IgG anti-FHOD3 Santa Cruz Biotechnology G-5, sc-374601 Used at 1:100 Sequencebased reagent SmartPool siRNA DIAPH1 GE Dharmacon E-010347-00-0005 Sequencebased reagent SmartPool siRNA DAAM1 GE Dharmacon E-012925-00-0005 Sequencebased reagent SmartPool siRNA FHOD3 GE Dharmacon E-023411-00-0005 Sequencebased reagent SmartPool siRNA MYH10 (NMIIB) GE Dharmacon E-023017-00-0010 Sequencebased reagent SmartPool siRNA MYH9 (NMIIA) GE Dharmacon E-007668-00-0005 Commercial assay or kit Rneasy Mini Kit Qiagen 74104 Continued on next page Fenix et al. eLife 2018;7:e42144.
Recombinant:Article Title: Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces
Article Snippet: Antibodies used in this study included the homemade affinity purified rabbit anti- T. gondii toxofilin [ ], affinity purified mouse anti- T. gondii RON4 antibodies [ ], mouse monoclonal anti -T. gondii P30 antibodies (Novocastra, Leica Biosystem, Nanterre, France), polyclonal rabbit anti-p34-Arc/ARPC2 (ref 07-227 batch 32474, Upstate, Millipore, Molsheim, France), mouse monoclonal anti-cortactin p80/p85 (clone 4 F11, ref 05-180, batch 28747, Upstate, Millipore, Molsheim, France), anti-non-muscle myosin II (ref M8064, Sigma-Aldrich, Lyon, France), mouse anti-Ty antibodies (ref 200-301-W45, Rockland Immunochemicals Inc., Limerick, PA, USA), anti-recombinant ROP2 serum (gift of J.F.
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35) (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis.
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35)23 (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Codependencies of mTORC1 signaling and endolysosomal actin structures
Article Snippet: 488 Goat Molecular Probes (A21206) 1/200 Anti-mouse Cy3 Donkey Jackson ImmunoResearch (715-165-151) 1/500 Anti-rabbit Cy3 Donkey Molecular Probes (A21206) 1/800 Anti-chicken Alexa Fluor 488 Donkey Molecular Probes (A11039) 1/300 Table S4.
Article Title: MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes
Article Snippet: The other antibodies used in this study were ARPC2/p34-Arc rabbit polyclonal (Millipore; 07–227, 1:1,000 dilution), ARPC5 mouse monoclonal (Synaptic Systems; 305011, clone 323H3, 1:1,000 dilution), Arp2 rabbit monoclonal (Abcam; ab129018, clone EPR7979, 1:1,000 dilution), ARPC5L rabbit monoclonal (Abcam; ab169763, clone EPR10274, 1:1,000 dilution), ARPC1A rabbit polyclonal (Sigma; HPA004334, 1:250 dilution), Arp3 mouse monoclonal (Sigma; A5979, clone FMS338, 1:1000 dilution), ARPC1B rabbit polyclonal (Bethyl Laboratories; A302-781, 1:1,000 dilution), ARPC3 mouse monoclonal (BD Transduction Laboratories; 612234, clone 26/p21-Arc, 1:1,000 dilution), ARPC4 rabbit polyclonal (Sigma; SAB1100901, 1:1,000 dilution), β-actin mouse monoclonal (Sigma; A5316, clone AC-74, 1:1,000 dilution), Vinculin mouse monoclonal (Sigma; V4505, 1:1000 dilution), coronin 1C rabbit polyclonal (Invitrogen; PA5-21775, 1:500 dilution), α-Actinin mouse monoclonal (Sigma; A5044, 1:1,000 dilution), GAPDH mouse monoclonal (Santa Cruz Biotechnology; sc-32233, 1:1,000 dilution), cortactin mouse monoclonal (Millipore; 05–180, clone 4F11, 1:1,000 dilution), MICAL1 rabbit polyclonal (Proteintech; 14818–1-AP, 1:1,000 dilution), GFP mouse monoclonal (custom made by Cancer Research UK; 1:1,000 dilution).
Article Title: Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes
Article Snippet: Antibody rabbit polyclonal IgG anti-NMIIA Biolegend P909801 Used at 1:1000 Antibody rabbit polyclonal IgG anti-NMIIB Cell Signaling P3404S Used at 1:200 Antibody rabbit polyclonal IgG anti-NMIIB Biolegend 909901 Used at 1:200 Antibody mouse monoclonal IgG anti-bCMII Iowa Hybridoma Bank A4.1025 Used at 1:2 Antibody rabbit polyclonal IgG anti-a-actinin-2 Sigma-Aldrich clone EA-53 Used at 1:200 Antibody rabbit polyclonal IgG anti-p34-Arc/ArpC2 (Arp2/3 complex) Millipore Sigma 07–227 Used at 1:100 Antibody rabbit polyclonal IgG anti-DAAM1 Bethyl Laboratories HPA026605 Used at 1:100 Antibody rabbit polyclonal IgG anti-DIAPH1 Sigma-Aldrich A300-078A Used at 1:100 Antibody mouse monoclonal IgGanti-cardiac troponin T Santa Cruz Biotechnology CT3, sc-20025 Used at 1:50 Antibody goat anti mouse IgG Alexa Fluor 568 ThermoFisher Scientific A-11004 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 568 ThermoFisher Scientific A-11011 Used at 1:100 Antibody goat anti mouse IgG Alexa Fluor 488 ThermoFisher Scientific AA28175 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 647 ThermoFisher Scientific A-21244 Used at 1:100 Antibody goat anti mouse IgM Alexa Fluor 488 ThermoFisher Scientific A-21042 Used at 1:100 Antibody mouse monoclonal IgG anti-FHOD3 Santa Cruz Biotechnology G-5, sc-374601 Used at 1:100 Sequencebased reagent SmartPool siRNA DIAPH1 GE Dharmacon E-010347-00-0005 Sequencebased reagent SmartPool siRNA DAAM1 GE Dharmacon E-012925-00-0005 Sequencebased reagent SmartPool siRNA FHOD3 GE Dharmacon E-023411-00-0005 Sequencebased reagent SmartPool siRNA MYH10 (NMIIB) GE Dharmacon E-023017-00-0010 Sequencebased reagent SmartPool siRNA MYH9 (NMIIA) GE Dharmacon E-007668-00-0005 Commercial assay or kit Rneasy Mini Kit Qiagen 74104 Continued on next page Fenix et al. eLife 2018;7:e42144.
Sequencing:Article Title: Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces
Article Snippet: Antibodies used in this study included the homemade affinity purified rabbit anti- T. gondii toxofilin [ ], affinity purified mouse anti- T. gondii RON4 antibodies [ ], mouse monoclonal anti -T. gondii P30 antibodies (Novocastra, Leica Biosystem, Nanterre, France), polyclonal rabbit anti-p34-Arc/ARPC2 (ref 07-227 batch 32474, Upstate, Millipore, Molsheim, France), mouse monoclonal anti-cortactin p80/p85 (clone 4 F11, ref 05-180, batch 28747, Upstate, Millipore, Molsheim, France), anti-non-muscle myosin II (ref M8064, Sigma-Aldrich, Lyon, France), mouse anti-Ty antibodies (ref 200-301-W45, Rockland Immunochemicals Inc., Limerick, PA, USA), anti-recombinant ROP2 serum (gift of J.F.
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35) (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis.
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35)23 (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Codependencies of mTORC1 signaling and endolysosomal actin structures
Article Snippet: 488 Goat Molecular Probes (A21206) 1/200 Anti-mouse Cy3 Donkey Jackson ImmunoResearch (715-165-151) 1/500 Anti-rabbit Cy3 Donkey Molecular Probes (A21206) 1/800 Anti-chicken Alexa Fluor 488 Donkey Molecular Probes (A11039) 1/300 Table S4.
Article Title: MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes
Article Snippet: The other antibodies used in this study were ARPC2/p34-Arc rabbit polyclonal (Millipore; 07–227, 1:1,000 dilution), ARPC5 mouse monoclonal (Synaptic Systems; 305011, clone 323H3, 1:1,000 dilution), Arp2 rabbit monoclonal (Abcam; ab129018, clone EPR7979, 1:1,000 dilution), ARPC5L rabbit monoclonal (Abcam; ab169763, clone EPR10274, 1:1,000 dilution), ARPC1A rabbit polyclonal (Sigma; HPA004334, 1:250 dilution), Arp3 mouse monoclonal (Sigma; A5979, clone FMS338, 1:1000 dilution), ARPC1B rabbit polyclonal (Bethyl Laboratories; A302-781, 1:1,000 dilution), ARPC3 mouse monoclonal (BD Transduction Laboratories; 612234, clone 26/p21-Arc, 1:1,000 dilution), ARPC4 rabbit polyclonal (Sigma; SAB1100901, 1:1,000 dilution), β-actin mouse monoclonal (Sigma; A5316, clone AC-74, 1:1,000 dilution), Vinculin mouse monoclonal (Sigma; V4505, 1:1000 dilution), coronin 1C rabbit polyclonal (Invitrogen; PA5-21775, 1:500 dilution), α-Actinin mouse monoclonal (Sigma; A5044, 1:1,000 dilution), GAPDH mouse monoclonal (Santa Cruz Biotechnology; sc-32233, 1:1,000 dilution), cortactin mouse monoclonal (Millipore; 05–180, clone 4F11, 1:1,000 dilution), MICAL1 rabbit polyclonal (Proteintech; 14818–1-AP, 1:1,000 dilution), GFP mouse monoclonal (custom made by Cancer Research UK; 1:1,000 dilution).
Article Title: Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes
Article Snippet: Antibody rabbit polyclonal IgG anti-NMIIA Biolegend P909801 Used at 1:1000 Antibody rabbit polyclonal IgG anti-NMIIB Cell Signaling P3404S Used at 1:200 Antibody rabbit polyclonal IgG anti-NMIIB Biolegend 909901 Used at 1:200 Antibody mouse monoclonal IgG anti-bCMII Iowa Hybridoma Bank A4.1025 Used at 1:2 Antibody rabbit polyclonal IgG anti-a-actinin-2 Sigma-Aldrich clone EA-53 Used at 1:200 Antibody rabbit polyclonal IgG anti-p34-Arc/ArpC2 (Arp2/3 complex) Millipore Sigma 07–227 Used at 1:100 Antibody rabbit polyclonal IgG anti-DAAM1 Bethyl Laboratories HPA026605 Used at 1:100 Antibody rabbit polyclonal IgG anti-DIAPH1 Sigma-Aldrich A300-078A Used at 1:100 Antibody mouse monoclonal IgGanti-cardiac troponin T Santa Cruz Biotechnology CT3, sc-20025 Used at 1:50 Antibody goat anti mouse IgG Alexa Fluor 568 ThermoFisher Scientific A-11004 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 568 ThermoFisher Scientific A-11011 Used at 1:100 Antibody goat anti mouse IgG Alexa Fluor 488 ThermoFisher Scientific AA28175 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 647 ThermoFisher Scientific A-21244 Used at 1:100 Antibody goat anti mouse IgM Alexa Fluor 488 ThermoFisher Scientific A-21042 Used at 1:100 Antibody mouse monoclonal IgG anti-FHOD3 Santa Cruz Biotechnology G-5, sc-374601 Used at 1:100 Sequencebased reagent SmartPool siRNA DIAPH1 GE Dharmacon E-010347-00-0005 Sequencebased reagent SmartPool siRNA DAAM1 GE Dharmacon E-012925-00-0005 Sequencebased reagent SmartPool siRNA FHOD3 GE Dharmacon E-023411-00-0005 Sequencebased reagent SmartPool siRNA MYH10 (NMIIB) GE Dharmacon E-023017-00-0010 Sequencebased reagent SmartPool siRNA MYH9 (NMIIA) GE Dharmacon E-007668-00-0005 Commercial assay or kit Rneasy Mini Kit Qiagen 74104 Continued on next page Fenix et al. eLife 2018;7:e42144.
Software:Article Title: Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces
Article Snippet: Antibodies used in this study included the homemade affinity purified rabbit anti- T. gondii toxofilin [ ], affinity purified mouse anti- T. gondii RON4 antibodies [ ], mouse monoclonal anti -T. gondii P30 antibodies (Novocastra, Leica Biosystem, Nanterre, France), polyclonal rabbit anti-p34-Arc/ARPC2 (ref 07-227 batch 32474, Upstate, Millipore, Molsheim, France), mouse monoclonal anti-cortactin p80/p85 (clone 4 F11, ref 05-180, batch 28747, Upstate, Millipore, Molsheim, France), anti-non-muscle myosin II (ref M8064, Sigma-Aldrich, Lyon, France), mouse anti-Ty antibodies (ref 200-301-W45, Rockland Immunochemicals Inc., Limerick, PA, USA), anti-recombinant ROP2 serum (gift of J.F.
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35) (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Myosin 1b and F-actin are involved in the control of secretory granule biogenesis.
Article Snippet: Primary antibodies used were monoclonal anti-synaptobrevin 2 (104211 from Synaptic system; 1:5,000); monoclonal α-tubulin (T5168 from Sigma; 1:5,000); rabbit polyclonal anti-CgA (EL-35)23 (1:500 for IF, 1:1,000 for Western blotting); goat polyclonal anti-CgA (sc-23556 from Santa Cruz Biotechnology inc; 1: 200); rabbit polyclonal anti-Myo1b (HPA 013607 from Sigma prestige antibodies; 1:200 for IF, 1:250 for Western blotting); mouse monoclonal anti-GM130 (610822 from BDBiosciences; 1:1,000); rabbit polyclonal anti-furin (Ab3467 from Abcam; 1:200); sheep polyclonal anti-human TGN46 (AHP500 from AbD serotec; 1:500); rabbit polyclonal anti-p34-Arc/ARPC2 (07-227 from Millipore; 1:400); goat anti-type III collagen (1330-01 from Southern Biotech; 1: 200).
Article Title: Codependencies of mTORC1 signaling and endolysosomal actin structures
Article Snippet: 488 Goat Molecular Probes (A21206) 1/200 Anti-mouse Cy3 Donkey Jackson ImmunoResearch (715-165-151) 1/500 Anti-rabbit Cy3 Donkey Molecular Probes (A21206) 1/800 Anti-chicken Alexa Fluor 488 Donkey Molecular Probes (A11039) 1/300 Table S4.
Article Title: MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes
Article Snippet: The other antibodies used in this study were ARPC2/p34-Arc rabbit polyclonal (Millipore; 07–227, 1:1,000 dilution), ARPC5 mouse monoclonal (Synaptic Systems; 305011, clone 323H3, 1:1,000 dilution), Arp2 rabbit monoclonal (Abcam; ab129018, clone EPR7979, 1:1,000 dilution), ARPC5L rabbit monoclonal (Abcam; ab169763, clone EPR10274, 1:1,000 dilution), ARPC1A rabbit polyclonal (Sigma; HPA004334, 1:250 dilution), Arp3 mouse monoclonal (Sigma; A5979, clone FMS338, 1:1000 dilution), ARPC1B rabbit polyclonal (Bethyl Laboratories; A302-781, 1:1,000 dilution), ARPC3 mouse monoclonal (BD Transduction Laboratories; 612234, clone 26/p21-Arc, 1:1,000 dilution), ARPC4 rabbit polyclonal (Sigma; SAB1100901, 1:1,000 dilution), β-actin mouse monoclonal (Sigma; A5316, clone AC-74, 1:1,000 dilution), Vinculin mouse monoclonal (Sigma; V4505, 1:1000 dilution), coronin 1C rabbit polyclonal (Invitrogen; PA5-21775, 1:500 dilution), α-Actinin mouse monoclonal (Sigma; A5044, 1:1,000 dilution), GAPDH mouse monoclonal (Santa Cruz Biotechnology; sc-32233, 1:1,000 dilution), cortactin mouse monoclonal (Millipore; 05–180, clone 4F11, 1:1,000 dilution), MICAL1 rabbit polyclonal (Proteintech; 14818–1-AP, 1:1,000 dilution), GFP mouse monoclonal (custom made by Cancer Research UK; 1:1,000 dilution).
Article Title: Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes
Article Snippet: Antibody rabbit polyclonal IgG anti-NMIIA Biolegend P909801 Used at 1:1000 Antibody rabbit polyclonal IgG anti-NMIIB Cell Signaling P3404S Used at 1:200 Antibody rabbit polyclonal IgG anti-NMIIB Biolegend 909901 Used at 1:200 Antibody mouse monoclonal IgG anti-bCMII Iowa Hybridoma Bank A4.1025 Used at 1:2 Antibody rabbit polyclonal IgG anti-a-actinin-2 Sigma-Aldrich clone EA-53 Used at 1:200 Antibody rabbit polyclonal IgG anti-p34-Arc/ArpC2 (Arp2/3 complex) Millipore Sigma 07–227 Used at 1:100 Antibody rabbit polyclonal IgG anti-DAAM1 Bethyl Laboratories HPA026605 Used at 1:100 Antibody rabbit polyclonal IgG anti-DIAPH1 Sigma-Aldrich A300-078A Used at 1:100 Antibody mouse monoclonal IgGanti-cardiac troponin T Santa Cruz Biotechnology CT3, sc-20025 Used at 1:50 Antibody goat anti mouse IgG Alexa Fluor 568 ThermoFisher Scientific A-11004 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 568 ThermoFisher Scientific A-11011 Used at 1:100 Antibody goat anti mouse IgG Alexa Fluor 488 ThermoFisher Scientific AA28175 Used at 1:100 Antibody goat anti rabbit IgG Alexa Fluor 647 ThermoFisher Scientific A-21244 Used at 1:100 Antibody goat anti mouse IgM Alexa Fluor 488 ThermoFisher Scientific A-21042 Used at 1:100 Antibody mouse monoclonal IgG anti-FHOD3 Santa Cruz Biotechnology G-5, sc-374601 Used at 1:100 Sequencebased reagent SmartPool siRNA DIAPH1 GE Dharmacon E-010347-00-0005 Sequencebased reagent SmartPool siRNA DAAM1 GE Dharmacon E-012925-00-0005 Sequencebased reagent SmartPool siRNA FHOD3 GE Dharmacon E-023411-00-0005 Sequencebased reagent SmartPool siRNA MYH10 (NMIIB) GE Dharmacon E-023017-00-0010 Sequencebased reagent SmartPool siRNA MYH9 (NMIIA) GE Dharmacon E-007668-00-0005 Commercial assay or kit Rneasy Mini Kit Qiagen 74104 Continued on next page Fenix et al. eLife 2018;7:e42144.
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